CMV pp65 – Recombinant Protein ( #130-091-824 #130-091-823 )

CMV pp65 – Recombinant Protein

Description

CMV pp65 Recombinant Protein is designed for the in vitro stimulation of pp65-specific CD4⁺ and CD8⁺ T cells. The resulting secretion of effector cytokines permits the subsequent detection of pp65-specific effector/memory T cells, for example, by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

Details

Background information
Human cytomegalovirus (CMV, HCMV) is a member of the herpes virus group. Between 50% and 85% of human adults are infected with CMV. Once infected, the virus persists in the organism. In healthy individuals, the infection is asymptomatic, but in immunocompromised patients CMV can cause severe disease. The pp65 protein (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. pp65 is an immunodominant target of CD4⁺ as well as CD8⁺ T cell responses to CMV. pp65-specific T cells predominantly produce cytokines such as IFN-γ, IL-2, and TNF-α.

Downstream applications

Detection of pp65-specific effector/memory T cells, for example, by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Restimulation of cells for the isolation of viable pp65-specific CD4⁺ and CD8⁺ T cells, for example, using the MACS^® Cytokine Secretion Assay – Cell Enrichment and Detection Kits.
Generation of pp65-specific CD4⁺ and CD8⁺ T cells from naive T cell populations, for example in immunotherapy or vaccination research.
Pulsing of antigen-presenting cells, e.g., as a model in research on antigen uptake and presentation.

Figure 1

Human PBMCs of a CMV⁺ donor were restimulated for 6 hours with or without CMV pp65 – Recombinant Protein. Brefeldin A was added after 2 hours. Cells were fixed, permeabilized, and pp65-specific cells were intracellularly stained with Anti-IFN-γ-PE. T cells were counterstained for CD4 and CD8 expression; IFN-γ production of lymphocytes is shown.

CD4⁺ T cells

A: Stimulated sample

B: Unstimulated control

CD8⁺ T cells

C: Stimulated sample

D: Unstimulated control