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- Easy: one-step isolation of untouched monocytes
- High-purity monocytes: perfectly suited for Mo-DC generation and other downstream applications
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| Overview |
| The Monocyte Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. For a more efficient isolation of all monocytes including CD16+ monocytes, the Pan Monocyte Isolation Kit, human ist recommended. |
| Details |
Detailed separation procedure
Non-monocytes are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies as well as Anti-Biotin MicroBeads. Highly enriched unlabeled monocytes are obtained by depletion of the magnetically labeled cells.
Downstream applications
Monocytes isolated with the Monocyte Isolation Kit II are used in studies where direct labeling of surface molecules is undesired. Untouched monocytes are also used for further isolation of specialized cell subsets such as activated monocytes. The most eminent use of isolated monocytes is their ex vivo differentiation into dendritic cells.1,2 |
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| Figure 1 |
| Isolation of untouched monocytes from PBMCs using the Monocyte Isolation Kit II, an LS Column, and a MidiMACS™ Separator. |
| PBMCs before separation |
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| Isolated untouched monocytes |
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| Figure 2 |
| Working scheme for the isolation of untouched monocytes using the Monocyte Isolation Kit II. |
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| Favorites / Prices |
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| Details |
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| Products |
| Monocyte Isolation Kit II, human |
- for 109 total cells
Download datasheet
130-091-153
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| References |
| 1. Hu et al. (2004) J. Exp. Med. 199: 1065-1075. |
| 2. Hafsi et al. (2004) J. Immunol. 173: 1249-1257. |
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