Monocyte Isolation Kit II
Description The Monocyte Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils, are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies against CD3, CD7, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A), as well as Anti-Biotin MicroBeads. Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells. For evaluation of MACS® Separations, staining with fluorochrome-conjugated Anti-Biotin antibodies (Anti-Biotin-FITC, -PE, or -APC) and CD14 antibodies is recommended. Applications Monocytes isolated with the Monocyte Isolation Kit II are used in studies where direct labeling of surface molecules could interfere with downstream applications. Untouched monocytes are also used for further isolation of specialized cell subsets such as activated monocytes. The most eminent use of isolated monocytes is their ex vivo differentiation into dendritic cells.1,2 Columns For depletion: LS or autoMACS™ Columns.		Figure 1 Isolation of untouched monocytes from PBMCs using the Monocyte Isolation Kit II, an LS Column, and a MidiMACS™ Separator. PBMCs before separation Isolated untouched monocytes Figure 2 Working scheme for the isolation of untouched monocytes using the Monocyte Isolation Kit II.