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- Efficient: maximum recovery without density gradient centrifugation
- Time-saving: true whole-blood application, without red blood cell lysis
- Gold standard: the most gentle way to isolate monocytes from whole blood
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| Overview |
| Whole Blood CD14 MicroBeads have been developed for rapid positive selection of CD14+ cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis. |
| Details |
Background information
The CD14 antigen belongs to the LPS receptor complex. It is strongly expressed on most monocytes and macrophages and weakly on neutrophils.
Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD14 MicroBeads to be subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
Downstream applications
Whole Blood MicroBeads allow the fast isolation of CD14+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safer handling of hazardous samples. The purified CD14+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2. |
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| Figure 1 |
| Monocytes were separated from whole blood using Whole Blood CD14 MicroBeads and the autoMACS® Separator with program Posseld2. Cells were fluorescently stained with CD14-FITC and CD45-PE. |
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| Favorites / Prices |
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| Details |
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| Products |
| Whole Blood CD14 MicroBeads, human |
| For research use only |
- for 40 mL whole blood
Download datasheet
130-090-879
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| References |
| 1. Köhl et al. (2003) Leukemia 17: 232–236. |
| 2. Meyer et al. (2006) Blood 109: 374–382. |
| 3. Willasch et al. (2010) Bone Marrow Transplantation 45: 181–189. |
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