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- Time-saving: one-step isolation or depletion of myeloid cells
- Flexible: tested to work with lymphoid tissue, bone marrow, and PBMCs
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| Overview |
| CD11b MicroBeads were developed for the isolation or depletion of human and mouse cells based on their CD11b expression. |
| Details |
Background information
In humans, CD11b is strongly expressed on myeloid cells, and weakly expressed on NK cells and some activated lymphocytes.
In mice, the CD11b antigen is expressed on monocytes/macrophages and to a lower extend on granulocytes, NK cells, CD5+ B1 cells and a subset of dendritic cells.
Downstream applications
In humans, CD11b MicroBeads can be used for positive selection or depletion of CD11b+ cells from PBMCs, bone marrow, and other lymphoid tissues. They are mostly used in combination with other MicroBeads for depletion of unwanted cells.
In mice, CD11b MicroBeads have been used to isolate CD11b+ cells from cell suspensions of spleen1, lymph node2, peritoneal cavity3, liver4, muscle tissue2, or blood5. They were also used in combination with other MicroBeads for the enrichment of mast cells from peritoneal cell suspensions.3
For the isolation of human monocytes CD14 MicroBeads or the Pan Monocyte Isolation Kit are recommended. |
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| Figure 1 |
| Separation of CD11b+ cells from human PBMCs using CD11b MicroBeads, an MS Column, and a MiniMACS™ Separator. |
| Isolation of human CD11b+ cells |
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| Figure 2 |
| Positive selection of murine CD11b+ cells from a splenocyte suspension using CD11b MicroBeads, an MS Column, and a MiniMACS Separator. |
| Mouse splenocytes before separation |
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| CD11b-depleted splenocytes |
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| Isolated CD11b+ mouse cells |
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| Favorites / Prices |
Request prices or add products to your favorite list.
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| Details |
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| Products |
| CD11b MicroBeads, human and mouse |
- for 1×109 total human cells, or for 2×109 total mouse cells
Download datasheet
130-049-601
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| CD11b MicroBeads, human and mouse – lyophilized |
- for 1×109 total human cells, or for 2×109 total mouse cells
Download datasheet
130-097-142
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| References |
| 1. Lohoff et al. (1997) Immunity 6: 681-689. |
| 2. Dupuis et al. (2000) J. Immunol. 165: 2850-2858. |
| 3. Chen et al. (1999) Immunol. 96: 544-550. |
| 4. Do et al. (1999) J. Biol. Chem. 274: 19587-19592 |
| 5. Mathy et al. (2000) J. Immunol. 164: 4429-4432 |
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