| Description |
| The NK Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched NK cells from human PBMCs. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and the NK Cell MicroBead Cocktail. Isolation of highly pure NK cells is achieved by depletion of magnetically labeled cells. |
| Applications |
| NK cells isolated with the NK Cell Isolation Kit are used in studies where direct labeling of surface molecules could interfere with downstream applications. Untouched NK cells are also used for studies on induction of NK cell proliferation or cytotoxic and cytolytic activity, analysis of “self/non-self” discrimination by NK cells, studies on perforin, granzyme, and cytokine expression in NK cells as well as the analysis of the functional role of NK cell surface receptors. |
| |
| Figure 1 |
| Isolation of untouched human NK cells from PBMCs by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. |
| A: PBMC before separation |
 |
| B: Isolated NK cells |
 |
|
|
