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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD56+CD16+ NK Cell Isolation Kit
Description
The CD56+CD16+ NK Isolation Kit was developed for the isolation of CD56dimCD16+ NK cells from PBMCs by sequential sorting. The CD56dim NK cell subset is highly cytotoxic even without stimulation, but has little to no cytokine production.
Details
Background information
NK cells represent a highly specialized lymphocyte population of the innate immune system playing an important role in early immune response.
NK cells can be divided into several subsets according to function and phenotype. CD56+CD16 NK cells represent the majority of all NK cells in the blood. They express killer immunoglobulin-like receptors (KIRs) and CD16 and, accordingly, show strong cell-mediated cytotoxicity as well as antibody-dependent cell-mediated cytotoxicity (ADCC).

Detailed separation procedure
The isolation of CD56+CD16+ NK cells is performed in a two-step procedure. First, non-NK cells are labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads und subsequently depleted by separation over a MACS® Column, which is placed in the magnetic field of a MACS Separator. In the second step, the target cells are labeled with CD16 MicroBeads and isolated from the pre-enriched cell fraction by positive selection over a second column.

Downstream applications
Cells isolated using the CD56+CD16+ NK Cell Isolation Kit can be used for the phenotypical and functional characterization of NK cell subsets, for the analysis of receptor expression, cytokine secretion, or cell-cell interaction with other cells of the innate and adaptive immune system.2 Furthermore, cells can also be used to study the role of NK cells in diseases, e.g., hepatitis infection or cancer, as well as during pregnancy.
Columns
For the first magnetic separation (depletion): LS or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
 
Figure 1
CD56+CD16+ NK cells were isolated from human PBMCs using the CD56+CD16+ NK Cell Isolation Kit, an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. Cells were fluorescently stained with CD16-FITC and CD56-PE.
A: PBMCs before magnetic separation
B: Enriched NK cells after depletion of non-NK cells
C: Isolated CD56+CD16+ NK cells
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Details
Products
CD56+CD16+ NK Cell Isolation Kit, human
- for 2x109 total cells
Download datasheet
130-092-660
Qty.:
 

Related products
CD16 Antibodies
CD56 Antibodies
References
1. Deniz, G. et al. (2002) Eur. J. Immunol. 32: 879–884.
2. Hagberg, N. et al. (2011) J. Immunol. 186:5085-5094.
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