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| Whole Blood CD56 MicroBeads |
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| Description |
Whole Blood CD56 MicroBeads were developed for the rapid positive selection of CD56+ cells directly from whole blood or bone marrow, minimizing hands-on time and maximizing yield of target cells. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.
Whole Blood MicroBeads in combination with the autoMACS® Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples. |
| Details |
Background information
The CD56 antigen is expressed by most NK cells and a small subset of T cells. Upon activation of NK cells, the surface expression of CD56 is increased.
Detailed separation procedure
Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD56 MicroBeads to be subsequently separated with the autoMACS® Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
Downstream applications
CD56+ cells isolated using CD56 Whole Blood MicroBeads are ideally suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2. |
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| Figure 1 |
| CD56+ cells were separated from whole blood using Whole Blood CD56 MicroBeads and the autoMACS Separator with program Posseld2. Cells were stained with CD56-PE and CD3-FITC. |
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| Favorites / Prices |
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| Details |
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| Products |
| Whole Blood CD56 MicroBeads, human |
| For research use only |
- for 40 mL whole blood
Download datasheet
130-090-875
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| References |
| 1. Köhl et al. (2003) Leukemia 17: 232–236. |
| 2. Meyer et al. (2006) Blood 109: 374–382. |
| 3. Willasch et al. (2010) Bone Marrow Transplantation 45: 181–189. |
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