Whole Blood CD56 MicroBeads
Description Whole Blood CD56 MicroBeads were developed for automated positive selection of CD56+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD56 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator. The CD56 antigen is expressed by most NK cells and a T cell subset. Upon activation of NK cells, the surface expression of CD56 is increased. Applications Whole Blood MicroBeads and the autoMACS™ Pro Separator or the autoMACS Separator allow fast sample processing under standardized conditions. This combination further allows easy and safe handling of hazardous samples. The purified CD56+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2. For more information on MACS® Whole Blood MicroBeads click here. Separation program autoMACS™ Pro Separator: Posselwb (0.25–15 mL sample volume). autoMACS Separator: Posseld2 (0.25–3 mL sample volume).		Figure 1 CD56+ cells were separated from whole blood using Whole Blood CD56 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were stained with CD56-PE and CD3-FITC. Figure 2 Cell isolation by using Whole Blood MicroBeads and the autoMACS™ Pro Separator or the autoMACS Separator.