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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD56+CD57+ NK Cell Isolation Kit
Description
The CD56+CD57+ NK Cell Isolation Kit was developed for the isolation of CD56+CD57+ NK cells and T cell subsets from human PBMCs or other single-cell suspensions by sequential sorting.
Details
Detailed separation procedure
In a first step, CD56+ cells are magnetically labeled with CD56 MultiSort MicroBeads and isolated by positive selection. After the release of the magnetic particles, the CD56+CD57+ target cells are magnetically labeled with CD57 MicroBeads to be positively selected a second time.
Columns
MS, LS, or autoMACS® Columns.
 
CD56+CD57+ cells were separated from human PBMCs using the CD56+CD57+ NK Cell Isolation Kit and an LS Column in a MidiMACS™ Separator for the first positive selection, followed by the separation over an MS Column in a MiniMACS™ Separator. Cells were fluorescently stained with CD56-APC and CD57-PE.
A: PBMCs before separation
B: Isolated CD56+CD57+ cells
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Details
Products
CD56+CD57+ NK Cell Isolation Kit, human
- for up to 109 total cells
Download datasheet
130-093-395
Qty.:
 

Related products
CD56 antibodies
CD57 antibodies
Propidium Iodide Solution (#130-093-233)
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