ProCatch Glutathione Resin
ProCatch Glutathione Resin is a new affinity chromatography material to purify Glutathione binding proteins such as Glutathione S-Transferase (GST). It is optimized for medium to large scale protein isolation from prokaryotic or eukaryotic, for example insect cells using a low-pressure, gravity-flow or batch procedure. The high affinity and specificity of the GST - Glutathione interaction enables a very fast and efficient purification of native proteins. For small-scale, highly pure protein isolation, the μMACS™ GST Isolation Kit is recommended.		Figure 1 Purification of a 30 kDa glutathione-binding protein with ProCatch Glutathione Resin A: Marker (lane 1), lysate from cell extract (lane 2), flow-through (lane 3), first eluate (lane 4), second eluate (lane 5), third eluate (lane 6) and eluate after boiling the resin (lane 7) were loaded onto a Coomassie-stained SDS gel. B: Low pressure column purification protocol. Peak 1 defines the non-bound protein fraction (flow-through). Peak 2 corresponds to the eluted glutathione-binding protein. Proteins leaving the column were monitored by measuring absorbance at 280 nm. A: Comassie-stained SDS gel B: Chromatogram corresponding to SDS gel