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| ProCatch Glutathione Resin |
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| ProCatch Glutathione Resin is a new affinity chromatography material to purify Glutathione binding proteins such as Glutathione S-Transferase (GST). It is optimized for medium to large scale protein isolation from prokaryotic or eukaryotic, for example insect cells using a low-pressure, gravity-flow or batch procedure. The high affinity and specificity of the GST - Glutathione interaction enables a very fast and efficient purification of native proteins. For small-scale, highly pure protein isolation, the μMACS™ GST Isolation Kit is recommended. |
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| Figure 1 |
Purification of a 30 kDa glutathione-binding protein with ProCatch Glutathione Resin
A: Marker (lane 1), lysate from cell extract (lane 2), flow-through (lane 3), first eluate (lane 4), second eluate (lane 5), third eluate (lane 6) and eluate after boiling the resin (lane 7) were loaded onto a Coomassie-stained SDS gel.
B: Low pressure column purification protocol. Peak 1 defines the non-bound protein fraction (flow-through). Peak 2 corresponds to the eluted glutathione-binding protein. Proteins leaving the column were monitored by measuring absorbance at 280 nm. |
| A: Comassie-stained SDS gel |
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| B: Chromatogram corresponding to SDS gel |
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