a-Hyb™ Hybridization Station
The a-Hyb™ Hybridization Station is a fully automated system for processing of diverse microarrays and in situ hybridization. Fast and reproducible results due to active circulation The unique active circulatory system results in an even distribution of liquid over the surface of the slides and accelerates diffusion-controlled processes. This allows to shorten processing times to significantly less than 50% compared to many manual protocols. Combined with a tight temperature control, the active circulation also leads to greater reproducibility and reliability of the assays. Suitable for high-throughput settings Using a microtiterplate format slide carrier, the a-Hyb Hybridization Station facilitates the individual handling of four standard slides in parallel. The a-Hyb Hybridization Station can be combined with standard laboratory robot systems enabling its integration into fully automated microarray processing. Easy protocol adaption The software offers a highly flexible protocol structure. Users can choose from standard actions like incubation, temperature change, washing, and drying and can combine these steps suiting their individual applications. In addition, temperature and media can be selected independently for each slide. Thereby, protocols can easily be adapted and optimized. Open system for a multitude of applications Using the a-Hyb™ Station leads to increased sensititvity or decreased processing times in many cases, e.g.: miRNA analysis microarrays with doubled sensitivity in situ hybridization in 8 hours instead of 2,5 days Agilent arrayCGH in 22 instead of 42 hours Protocols for a wide variety of applications are available, e.g.: cDNA and oligonucleotide gene expression microarrays, e.g. PIQOR™ Microarrays arrayCGH for Agilent and other platforms microRNA microarrays, e.g. miRXplore™ Micorarrays Antibody and other protein microarrays Tissue microarrays In situ hybridizations, etc. Please contact us for a list of available protocols. Further information a-Hyb™ Hybridization Station flyer [PDF; 279,1 KB]		Figure 1 Fully automated a-Hyb™ Hybridization Station Figure 2 A twofold increase in sensitivity was achieved by using the a-Hyb™ Hybridization Station. microRNA from 2 µg of total liver RNA were hybridized to the miRXplore™ Universal Reference using the a-Hyb Station (A) or a manual hybridization protocol (B), respectively. A B Figure 3 Faster results—8 hours instead of 2.5 days Manual in situ hybridization: 16 hours. Overall processing time: 2,5 days. Manual in situ hybridization after 2 hours. a-Hyb™ Station employing active circulation hybridization: 2 hours. Overall processing time: 8 hours.

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