| Description |
The CD137 MicroBead Kit is an indirect magnetic labeling system for the enrichment of human CD137+ cells. CD137+ cells are indirectly magnetically labeled with CD137-PE and Anti-PE MicroBeads. CD137-PE recognizes the CD137 (4-1BB) antigen, a 30 kDa glycoprotein of the tumor necrosis factor (TNF) receptor superfamily. CD137 is mainly expressed on activated CD4+ and CD8+ T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4+ or CD8+ T cells, and NK cells.
Recently, CD137 has been described to be a suitable marker for antigen-specific activation of human CD8+ T cells, as CD137 is not expressed on resting CD8+ T cells and its expression is reliably induced after 24 hours of stimulation.1 In a previous study, CD137-expressing T cells were targeted for selective depletion of alloreactive T cells from antileukemic and antitumor donor T cell lines.2 |
| Applications |
The CD137 MicroBead Kit was developed for positive selection of CD137+ cells from human PBMCs, for example, for phenotypical and functional characterization of activated CD137+ T cells.
For the isolation of activated antigen-specific CD8+ T cells, the CD137 MicroBead Kit can be combined with the CD8+ T Cell Isolation Kit II. |
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| Figure 1 |
| PBMCs of a CMV+ donor were stimulated overnight with PepTivator – CMV pp65 or left untreated. The responding CD137+ T cells were separated using the CD137 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Cells were additionally stained with CD8-FITC. |
| Stimulated sample |
| A: Before separation |
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| B: Isolated CD137+ cells |
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| Unstimulated control |
| C: Before separation |
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| D: Isolated CD137+ cells |
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