Naive CD8+ T Cell Isolation Kit
Description The Naive CD8+ T Cell Isolation Kit was developed for the isolation of human naive CD8+ T cells from PBMCs. The isolation of naive CD8+ T cells is performed in a two-step procedure. First, naive T cells are enriched by depletion of non-naive T cells and NK cells, which are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. In a second step, the enriched naive T cells are labeled with CD8 MicroBeads for subsequent positive selection of the CD8+ naive T cells. Applications Naive CD8+ T cells isolated with the Naive CD8+ T Cell Isolation Kit can be used for functional T cell studies, e.g., studies on signal transduction or cytokine expression after activation of the naive CD8+ T cells. Columns For depletion of non-naive T cells: LD or autoMACS™ Columns. For positive selection of CD8+ cells: MS or autoMACS Columns.		Figure 1 Naive CD8+ T cells were isolated from human PBMCs by using the Naive CD8+ T Cell Isolation Kit, an LD Column and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. Cells were stained with CD8-PE-Cy7, CD56-PE, CD57-PE, CD45RO-PE, CD45RA-APC, and Anti-CCR7-FITC. Before separation Gated on viable CD8+CD56–CD57–CD45RO– cells Pre-enriched naive CD8+ T cells Gated on viable CD8+CD56–CD57–CD45RO– cells Isolated naive CD8+ T cells Gated on viable CD8+CD56–CD57–CD45RO– cells Figure 2 Isolation of naive CD8+ T cells using the Naive CD8+ T Cell Isolation Kit.