| Description |
CD25 MicroBeads II were developed for positive selection and depletion of CD25+ cells. CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4+ T cells, which has regulatory function.
The CD25 MicroBeads II recognize epitope A of the CD25 molecule.
For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS® CD25 Antibodies, which bind to epitope B of the CD25 antigen.
This product replaces CD25 MicroBeads (# 130-090-445). |
| Applications |
| CD25 MicroBeads II can be used for the depletion or positive selection of CD25+ cells from human peripheral blood, lymphatic tissues, or cultured cells. Isolation or depletion of CD25+ activated T cells, B cells, and monocytes can be achieved from in vitro antigen-, allogen-, or mitogen-stimulated lymphocytes. For isolation of CD25+ cell subsets, the CD25 MicroBeads II can be combined with MACS® Cell Isolation Kits or MultiSort Kits. The CD4+CD25+ Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4+CD25+ regulatory T cells. |
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| Figure 1 |
| Depletion of CD25+ cells from PBMCs using CD25 MicroBeads II, an LD Column, and a MidiMACS™ Separator. |
| PBMCs before separation |
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| CD25+ cell depleted fraction |
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