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| TCRγ/δ+ T Cell Isolation Kit |
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| Description |
| The TCRγ/δ+ T Cell Isolation Kit was developed for the isolation of untouched human γ/δ+ T cells from PBMCs. |
| Details |
Background information
TCRγ/δ is expressed in less than 10% of human peripheral blood T cells. During the immune response γ/δ T cells are able to recognize the antigen directly and not as peptide-MHC complex.
Detailed separation procedure
The non-γ/δ T cells are indirectly magnetically labeled with a cocktail of biotinylated monoclonal antibodies and Anti-Biotin MicroBeads. The magnetically labeled non-TCRγ/δ+ cells are retained in a MACS® Column in the magnetic field of a MACS Separator, while the unlabeled γ/δ T cells pass through the column.
Downstream applications
The TCRγ/δ+ T Cell Isolation Kit can be used for functional studies on γ/δ T cells when direct magnetic labeling of surface molecules could interfere with downstream applications, e.g., phenotypical and functional analysis such as studies on cytokine expression and receptor signaling, antigen recognition and cytotoxicity. |
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| Figure 1 |
| Untouched γ/δ T cells were isolated from PBMCs using the TCRγ/δ+ T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells were fluorescently stained with CD3-PE and Anti-TCRγ/δ-FITC. |
| A: Before separation |
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| B: Isolated TCRγ/δ+ T cells |
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| Details |
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| Products |
| TCRγ/δ+ T Cell Isolation Kit, human |
- for 109 total cells
Download datasheet
130-092-892
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