| Description |
The TCRγ/δ+ T Cell Isolation Kit was developed for the isolation of human γ/δ T cells from PBMCs by depletion of non-TCRγ/δ+ cells. The non-γ/δ T cells are indirectly magnetically labeled with a cocktail of biotinylated monoclonal antibodies and Anti-Biotin MicroBeads. The magnetically labeled non-TCRγ/δ+ cells are retained
in a MACS® Column in the magnetic field of a MACS Separator, while the unlabeled γ/δ T cells pass through the column.
TCRγ/δ is expressed in less than 10% of human peripheral blood T cells. During the immune response γ/δ T cells are able to recognize the antigen directly and not as peptide-MHC complex. |
| Applications |
| The TCR γ/δ+ T Cell Isolation Kit provides a fast and efficient way to isolate untouched γ/δ T cells. The TCRγ/δ+ T Cell Isolation Kit can be used for functional studies on γ/δ T cells when direct magnetic labeling of surface molecules could interfere with downstream applications, e.g., phenotypical and functional analysis such as studies on cytokine expression and receptor signaling, antigen recognition and cytotoxicity. |
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| Figure 1 |
| Untouched γ/δ T cells were isolated from PBMCs using the TCRγ/δ+ T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells were fluorescently stained with CD3-PE and Anti-TCRγ/δ-FITC. |
| A: Before separation |
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| B: Isolated TCRγ/δ+ T cells |
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