| Description |
The CD3+CD56+ NKT Cell Isolation Kit is a two-step magnetic labeling system for the isolation of CD3+CD56+ natural killer (NK) T cells from human PBMCs. In the first step, NK cells and monocytes are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted by separation over a MACS® Column. In the second step, CD3+CD56+ NKT cells are directly labeled with CD56 MicroBeads and isolated by positive selection from the pre-enriched NKT cell fraction. The magnetically labeled CD3+CD56+ NKT cells are retained in the column and eluted after removal of the column from the magnetic field.
NKT cells represent a subpopulation of T cells that possesses properties of NK cells. NKT cells can be stimulated through contact with antigen or by cytokines such as IL-12 to release large amounts of cytokines and to exert cytotoxic effects. NKT cells are a crucial part of the innate immune system and have an influence on the development of autoimmune diseases. They are also involved in tumor immunology as well as immunity against viruses, bacterial, fungal, and parasitic pathogens. |
| Applications |
| The CD3+CD56+ NKT Cell Isolation Kit can be used for a variety of studies such as cytokine production analysis after activation/stimulation, gene expression analysis of subsets, analysis of the functional role of CD3+CD56+ NKT cell surface receptors, cytotoxic and cytolytic activity. |
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