| Description |
| CD62L MicroBeads were developed for the magnetic isolation of lymphocyte subsets. The CD62L antigen is a 74 kDa glycoprotein and is a member of the selectin family of cell surface molecules, also referred to as L-selectin, LECAM-1, or LAM-1. CD62L binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. CD62L is important for homing of naive lymphocytes via the high endothelial venules to peripheral lymph nodes and Peyer's patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Most hematopoietic cells, including most peripheral blood B cells, T cells, monocytes, dendritic cells, granulocytes, and some myeloid cells from bone marrow and thymocytes, express CD62L. CD62L is continuously endoproteolytically cleaved from the cell surface of CD62L-expressing neutrophils and lymphocytes (shedding). Proteolysis is accelerated, e.g., after antigen activation of T cells. |
| Applications |
| CD62L MicroBeads were developed for positive selection or depletion of CD62L+ cells from PBMCs, body fluids (e.g. bronchial lavage), or single-cell suspensions from tissue (e.g. lymphoid or tumor tissue). CD62L+ T cells can also be selected from pre-enriched CD4+ or CD8+ T cells using the CD4+ T Cell Isolation Kit II or CD8+ T Cell Isolation Kit II. CD4+CD62L+ central memory T cells can be isolated from CD4+ memory T cells, which were pre-enriched with the Memory CD4+ T Cell Isolation Kit. |
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| Figure 1 |
| CD62L+ cells were separated from PBMCs using CD62L MicroBeads. For positive selection, CD62L+ cells were isolated using an MS Column and a MiniMACS™ Separator. For depletion of CD62L+ cells, the sample was separated over an LD Column in a MidiMACS™ Separator. Cells are fluorescently stained with CD62L-PE and CD4-FITC . |
| A: PBMCs before separation |
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| B: Depletion of CD62L+ cells |
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| C: Positive selection of CD62L+ cells |
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