| Description |
| The Naive CD4+ T Cell Isolation Kit is an indirect magnetic labeling system for the isolation of untouched naive T helper cells from human peripheral blood. For magnetic depletion of memory T cells, CD8+ T cells, B cells, NK cells, γ/δ T cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD16, CD19, CD56, CD36, CD123, anti-TCRγ/δ, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. The Naive CD4+ T Cell Isolation Kit provides a fast and efficient way to isolate untouched naive CD4+ T cells. |
| Applications |
| Naive CD4+ T cells isolated with the Naive CD4+ T Cell Isolation Kit are used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.1, 2 Untouched naive CD4+ T cells can also be used for further isolation of specific subsets. |
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| Figure 1 |
| Isolation of untouched naive CD4+ T helper cells from PBMCs using the Naive CD4+ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. |
| PBMCs before separation |
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| Untouched naive CD4+ T cells |
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| Figure 2 |
| Isolation of untouched naive T helper cells using the Naive CD4+ T Cell Isolation Kit. |
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