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Anti-TCRγ/δ MicroBead Kit 
Description
The Anti-TCRγ/δ MicroBead Kit is an indirect magnetic and fluorescent labeling system for the isolation of T cells expressing the γ/δ variant of the T cell receptor (TCRγ/δ). The Anti-TCRγ/δ MicroBead Kit consists of a hapten-conjugated Anti-TCRγ/δ antibody and FITC-conjugated Anti-Hapten MicroBeads. TCRγ/δ is expressed on up to 10% of the peripheral blood T cells.
Applications
The Anti-TCRγ/δ MicroBead Kit is a magnetic labeling system for the positive selection or depletion of TCRγ/δ-expressing T cells from peripheral blood, lymph nodes, thymus, spleen, and cultured cells. TCRγ/δ+ T cells isolated with the Anti-TCRγ/δ MicroBead Kit have been expanded and used for proliferation and cytotoxicity assays.1,2,3 Cutaneous γ/δ T cells, isolated from human skin using the Anti-TCRγ/δ MicroBead Kit, were cloned, expanded, and functionally characterized.4
Columns
For positive selection: MS, LS, XS, or autoMACSâ„¢ Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Isolation and depletion of TCRγ/δ+ cells from PBMCs using the Anti-TCRγ/δ MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
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Products
Anti-TCRγ/δ MicroBead Kit, human
for 109 total cells
Download data sheet
130-050-701
Qty:
 

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MACS References
1. Schilbach et al. (2000) J. Immunother. 23: 536-548.[866]
2. Schilbach et al. (2001) Blood 97: 2917-2918.[2287]
3. Rothenfusser et al. (2001) Eur. J. Immunol. 31: 3525-3534.[2010]
4. Ebert et al. (2006) J. Immunol. 176: 4331-4336.[8608]
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