| Description |
| CD45RA MicroBeads were developed for magnetic isolation of lymphocyte subsets. CD45RA is expressed on naive CD4+ and CD8+ T cells as well as on CD8+ effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA). |
| Applications |
| CD45RA MicroBeads are used for the positive selection or depletion of CD45RA+ cells. For isolation of CD45RA+ T cell subsets, the CD45RA MicroBeads can be combined with MACS® Cell Isolation Kits6,7 or CD4 and CD8 MultiSort Kits1,2. The CD45RA MicroBeads can also be used to isolate CD45RO+ cells from pre-sorted CD4+ or CD8+ T cells by depletion of CD45RA+ cells.3,4,5,7 Cells isolated with CD45RA MicroBeads can be used for various studies, such as cytokine expression or receptor signaling. |
| |
| Figure 1 |
| CD8+ cells were enriched from PBMCs by using the CD8 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RA MicroBeads. Naive CD8+CD45RA+ T cells were isolated by positive selection. |
| Pre-enriched CD8+ cells |
 |
| CD8+CD45RA+ cells |
 |
|
|
