| Description |
| CD27 MicroBeads were developed for the separation of cells, based on the expression of the CD27 antigen, which is a member of the tumor necrosis factor receptor family. CD27 is expressed on naive and memory CD8+ T cells, whereas effector T cells are negative for CD27. CD27 is also present on memory B cells and plasma cells but not on naive B cells. NK cells express CD27 as well. |
| Applications |
| CD27 MicroBeads can be used for the isolation of T cell subsets from human peripheral blood or lymphoid tissue. T cells can first be pre-selected using the CD8+ T Cell Isolation Kit II or the CD8 MultiSort Kit and subsequently separated according to CD27 expression using CD27 MicroBeads. CD27 MicroBeads can also be used for the isolation of CD19+CD27+ memory B cells. |
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| Figure 1 |
| Isolation of CD8+CD27– effector T cells and CD8+CD27+ T cells from human PBMCs. CD8+ T cell were pre-selected using the CD8+ T Cell Isolation Kit II followed by selection with CD27 MicroBeads. |
| A: Pre-selected CD8+ T cells |
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| B: CD8+CD27– effector T cells |
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| C: CD8+CD27+ T cells |
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