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| CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit |
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| Description |
| The CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit was developed for the isolation of CD45RA+ regulatory T cells from human PBMCs in a two-step procedure. |
| Details |
Background information
Regulatory CD4+CD25+ T cells isolated from the CD45RA+ naive T cell compartment were shown to be optimal for in vitro expansion. These expanded regulatory T cells maintained the FoxP3+ phenotype and their suppressive activity, whereas CD45RA– regulatory T cells from the memory T cell compartment lost FoxP3 expression and showed only modest suppressive activity.1
Detailed separation procedure
The isolation is performed in a two-step procedure. First, non-CD4+ and memory T cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS® Column. In the second step, the flow-through fraction of pre-enriched CD4+CD45RA+ T cells is labeled with CD25 MicroBeads for subsequent positive selection of CD4+CD25+CD45RA+ T cells.
Downstream applications
CD4+CD25+CD45RA+ regulatory T cells can be isolated from human PBMCs with this kit, for example, for further phenotypical or functional characterization and expansion. |
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| CD4+CD25+CD45RA+ regulatory T cells were isolated from human PBMCs by using the CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4+ lymphocytes (A, B). |
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| Details |
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| Products |
| CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kit, human |
- for 2×109 total cells
Download datasheet
130-093-631
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| References |
| 1. Hoffmann, P. et al. (2006) Blood 108: 4260–4267. |
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