CD4<sup>+</sup>CD25<sup>+</sup> Regulatory T Cell Isolation Kit ( #130-091-301 )

CD4⁺CD25⁺ Regulatory T Cell Isolation Kit

Description

The CD4⁺CD25⁺ Regulatory T Cell Isolation Kit was developed for the isolation of CD4⁺CD25⁺ cells from PBMCs. The kit contains a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion of non-CD4⁺ T cells, and CD25 MicroBeads for subsequent positive selection of the CD4⁺CD25⁺ cells. The kit also includes CD25-PE for quality control of the separations.
CD4⁺CD25⁺ T cells act as regulatory T cells upon activation through their T cell receptor. CD4⁺CD25⁺ regulatory T cells were originally discovered in mice, but a population with similar phenotypic and functional properties has also been characterized in humans. Regulatory CD4⁺CD25⁺ T cells seem to suppress harmful immunological reactions to self or foreign antigens.

Applications

The CD4⁺CD25⁺ Regulatory T Cell Isolation Kit has been used to investigate the role of CD4⁺CD25⁺regulatory T cells in autoimmune diseases^1,2, antitumor immunity^3-5, allergy⁶, and in infectious diseases⁷. For further characterization, CD4⁺CD25⁺regulatory T cells were isolated from patients suffering from rheumatoid arthritis¹ or multiple sclerosis², from acute T cell lymphoma patients⁴, from tumor biopsy specimens of non-Hodgkin lymphoma patients⁵ as well as from patients with atopic dermatitis⁶.

Columns

For depletion of non-CD4⁺ T cells: LD or autoMACS™ Columns. For positive selection of CD25⁺ cells: MS or autoMACS Columns.

Figure 1

Isolation of human peripheral blood CD4⁺CD25⁺ regulatory T cells using the CD4⁺CD25⁺ Regulatory T Cell Isolation Kit.

Pre-enriched CD4⁺ T cells after depletion of non-CD4⁺ cells

CD4⁺CD25⁺ T cells

Figure 2

Analysis of Foxp3 expression by CD4⁺CD25⁺ regulatory T cells, gated on CD4⁺ cells

Before separation

After separation

Figure 3

Analysis of CD127 expression by CD4⁺CD25⁺ regulatory T cells, gated on CD4⁺ cells.

Before separation

After separation