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navigationMACS® Cell Separation Reagents
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Biotec
for human cellsfor human cellsfor human cells
Stem and progenitor cellsStem and progenitor cellsStem and progenitor cellsStem and progenitor cells
T cellsT cellsT cellsT cells
Pan T cellsPan T cellsPan T cells>Pan T cells
CD4<sup>+</sup> T cellsCD4<sup>+</sup> T cellsCD4<sup>+</sup> T cells>CD4+ T cells
CD8<sup>+</sup> T cellsCD8<sup>+</sup> T cellsCD8<sup>+</sup> T cells>CD8+ T cells
Regulatory T cellsRegulatory T cellsRegulatory T cells>Regulatory T cells
Other T cell subsetsOther T cell subsetsOther T cell subsets>Other T cell subsets
NK cellsNK cellsNK cellsNK cells
B CellsB CellsB CellsB Cells
MonocytesMonocytesMonocytesMonocytes
Dendritic cellsDendritic cellsDendritic cellsDendritic cells
Antigen-presenting cellsAntigen-presenting cellsAntigen-presenting cellsAntigen-presenting cells
Granulocytes and myeloid cellsGranulocytes and myeloid cellsGranulocytes and myeloid cellsGranulocytes and myeloid cells
LeukocytesLeukocytesLeukocytesLeukocytes
Erythroid cellsErythroid cellsErythroid cellsErythroid cells
Megakaryocytes and plateletsMegakaryocytes and plateletsMegakaryocytes and plateletsMegakaryocytes and platelets
Endothelial cellsEndothelial cellsEndothelial cellsEndothelial cells
Epithelial cellsEpithelial cellsEpithelial cellsEpithelial cells
FibroblastsFibroblastsFibroblastsFibroblasts
Neural cellsNeural cellsNeural cellsNeural cells
Cytokine-producing cellsCytokine-producing cellsCytokine-producing cellsCytokine-producing cells
Tumor cellsTumor cellsTumor cellsTumor cells
Fc receptor blockingFc receptor blockingFc receptor blockingFc receptor blocking
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Biotec
for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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Biotec
for mouse cellsfor mouse cellsfor mouse cells
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Biotec
for rat cellsfor rat cellsfor rat cells
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Biotec
for indirect magnetic labelingfor indirect magnetic labelingfor indirect magnetic labeling
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Biotec
for apoptotic and dead cellsfor apoptotic and dead cellsfor apoptotic and dead cells
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Biotec
for isolation of mitochondriafor isolation of mitochondriafor isolation of mitochondria
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Biotec
MACS® Cell Separation Columns
MACS® Separators
autoMACS® Cell Separation
MACS® Cell Separation Buffers
MACS® Accessories
MACS® Products for sample preparation
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CD4+CD25+ Regulatory T Cell Isolation Kit
Description
The CD4+CD25+ Regulatory T Cell Isolation Kit was developed for the isolation of CD4+CD25+ cells from PBMCs. The kit contains a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion of non-CD4+ T cells, and CD25 MicroBeads for subsequent positive selection of the CD4+CD25+ cells.
Regulatory CD4+CD25+ T cells are suppressor cells that neutralize other immune cells by various mechanisms. Their characteristic marker is the transcription factor FoxP3. Regulatory CD4+CD25+ T cells seem to suppress harmful immunological reactions to self or foreign antigens.
Applications
The CD4+CD25+ Regulatory T Cell Isolation Kit has been used to investigate the role of CD4+CD25+ regulatory T cells in autoimmune diseases1,2, antitumor immunity3–6, allergy7, and in infectious diseases8. For further characterization, CD4+CD25+ regulatory T cells were isolated from patients suffering from rheumatoid arthritis1 or multiple sclerosis2, from acute T cell lymphoma patients4, from tumor biopsy specimens of non-Hodgkin lymphoma patients5, after dendritic cell vaccination9 as well as from patients with atopic dermatitis7.
Columns
For depletion of non-CD4+ T cells: LD or autoMACS® Columns. For positive selection of CD25+ cells: MS or autoMACS Columns.
 
Figure 1
CD4+CD25+ regulatory T cells were isolated from human PBMCs. The cells were fluorescently stained as indicated and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression except for the CD25-APC vs. CD4 (VIT4)-VioBlue dot plot.
Before separation
A
B
Isolated CD4+CD25+ regulatory T cells
A
B
C
Pre-enriched CD4+ T cells
A
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Products
CD4+CD25+ Regulatory T Cell Isolation Kit, human
- for 109 total cells
Download datasheet
130-091-301
Qty.:
 

Related products
CD4 antibodies
MACS® Cell Analysis Reagents
Anti-FoxP3 antibodies
CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit II
Treg Suppression Inspector, human
References
1. Ehrenstein et al. (2004) J. Exp. Med. 200: 277-285.
2. Hong et al. (2006) Proc. Natl. Acad. Sci. USA 103: 5024-5029.
3. Nishikawa et al. (2006) J. Immunol. 176: 6340-6346.
4. Karube et al. (2004) Br. J. Haematol. 126: 81-84.
5. Yang et al. (2006) Blood 107: 3639-3646.
6. Mougiakos et al. (2009) Blood 113: 3542-3545.
7. Ou et al. (2004) J. Allergy Clin. Immunol. 113: 756-763.
8. Xu et al. (2006) J. Immunol. 177: 739-747.
9. Schwaab et al. (2009) Clin. Cancer Res. 15: 4986-4992.
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