CD4+CD25+ Regulatory T Cell Isolation Kit
Description The CD4+CD25+ Regulatory T Cell Isolation Kit was developed for the isolation of CD4+CD25+ cells from PBMCs. The kit contains a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads for depletion of non-CD4+ T cells, and CD25 MicroBeads for subsequent positive selection of the CD4+CD25+ cells. Regulatory CD4+CD25+ T cells are suppressor cells that neutralize other immune cells by various mechanisms. Their characteristic marker is the transcription factor FoxP3. Regulatory CD4+CD25+ T cells seem to suppress harmful immunological reactions to self or foreign antigens. Applications The CD4+CD25+ Regulatory T Cell Isolation Kit has been used to investigate the role of CD4+CD25+ regulatory T cells in autoimmune diseases1,2, antitumor immunity3–6, allergy7, and in infectious diseases8. For further characterization, CD4+CD25+ regulatory T cells were isolated from patients suffering from rheumatoid arthritis1 or multiple sclerosis2, from acute T cell lymphoma patients4, from tumor biopsy specimens of non-Hodgkin lymphoma patients5, after dendritic cell vaccination9 as well as from patients with atopic dermatitis7. Columns For depletion of non-CD4+ T cells: LD or autoMACS® Columns. For positive selection of CD25+ cells: MS or autoMACS Columns.		Figure 1 CD4+CD25+ regulatory T cells were isolated from human PBMCs. The cells were fluorescently stained as indicated and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression except for the CD25-APC vs. CD4 (VIT4)-VioBlue dot plot. Before separation A B Isolated CD4+CD25+ regulatory T cells A B C Pre-enriched CD4+ T cells A

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