CD127 MicroBead Kit
Description The CD127 MicroBead Kit has been developed for the magnetic isolation or depletion of CD127+ cells. CD127, the α-chain of the IL-7 receptor, is a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).1 CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.2,3 On regulatory T cells CD127 is absent4 and its expression is inversely correlated with FoxP3 expression and suppressive function.5,6 CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.1 Applications Isolation or depletion of CD127+ T cells. Depletion of CD127+ T cells for the enrichment of regulatory T cells. Human regulatory T cells can be isolated, for example, by using the CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit, human. Columns For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, CS, D, or autoMACS Columns.		Figure 1 CD127+ cells were isolated from human PBMCs using the CD127 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4 (VIT4)-FITC and CD127-PE and analyzed by using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. A: Before separation B: Negative fraction C: Isolated CD127+ cells

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