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| CD25+CD49d– Regulatory T Cell Isolation Kit |
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| Description |
| The CD25+CD49d- Regulatory T Cell Isolation Kit was developed for the isolation of CD4+CD25+CD49d- regulatory T cells from human PBMCs in a two-step procedure. |
| Details |
Background information
CD49d is the α-chain of the integrin VLA-4 and is present on the majority of pro-inflammatory effector cells but absent on FoxP3+ cells. Consequently, the depletion of CD49d+ cells is a useful tool to isolate highly pure populations of CD25+CD49d– regulatory T cells.
Detailed separation procedure
The isolation of CD25+CD49d– regulatory T cells is performed in a two-step procedure. First, CD8+ and CD49d+ cells are magnetically labeled with a cocktail of CD8 and CD49d MicroBeads for depletion. In the second step, CD49d– regulatory T cells are labeled with CD25 MicroBeads and isolated by positive selection from the pre-enriched T cell fraction.
The depletion of CD49d+ cells removes contaminating CD25+ effector T cells1, thus providing highly pure populations of regulatory T cells. |
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| Details |
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| Products |
| CD25+CD49d– Regulatory T Cell Isolation Kit, human |
- for 109 total cells
Download datasheet
130-094-551
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| References |
| 1. Kleinewietfeld et al. (2009) Blood 113: 827–836. |
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