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| CD8+CD57+ T Cell Isolation Kit |
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| Description |
| The CD8+CD57+ T Cell Isolation Kit was developed for the isolation of CD57+ cytotoxic T cells by sequential sorting from human PBMCs or other single-cell suspensions. |
| Details |
Background information
The CD57 antigen has been shown to be expressed on late-stage CD8+ effector cells.1 CD57 expression is also increased on proliferation-incompentent or chronically activated CD8+ T cells in persistent viral infections, such as HIV.2
Detailed separation procedure
The separation of CD8+CD57+ T cells is performed in a two-step procedure. In a first step, CD8+ cells are magnetically labeled with CD8 MultiSort MicroBeads and isolated by positive selection. After the release of the magnetic particle, the CD8+CD57+ target cells are magnetically labeled with CD57 MicroBeads to be positively selected a second time. |
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| Figure 1 |
| CD8+CD57+ cells were separated from human PBMCs using the CD8+CD57+ T Cell Isolation Kit and an LS Column in a MidiMACS™ Separator for the first positive selection, followed by the separation over an MS Column in a MiniMACS™ Separator. Cells were fluorescently stained with CD8-FITC and CD57-PE. |
| A: Before separation |
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| B: After separation |
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| Details |
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| Products |
| CD8+CD57+ T Cell Isolation Kit, human |
- for 109 total cells
Download datasheet
130-093-396
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| References |
| 1. Brenchley et al. (2003) Blood 101: 2711–2720. |
| 2. Palmer et al. (2005) J. Immunol. 175: 8415–8423. |
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