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| Description |
| The CD8 MultiSort Kit is a direct magnetic labeling system designed for the isolation of distinct CD8+ T and NK cell subsets from human PBMCs, bone marrow, cell cultures, or single-cell suspensions from tissue. The CD8+ cells are isolated with CD8 MultiSort MicroBeads. Then the magnetic particles are enzymatically released. The selected CD8+ cells can again be magnetically labeled and sorted for a second antigen. |
| Details |
Downstream applications
The CD8 MultiSort Kit can be used to isolate specific CD8+ T cell or NK cell subsets. The selected CD8+ cell population can be sorted according to a second marker of interest, e.g., CD45RA, CD45RO, CD16, CD251, CD272, CD28, CD30, CD56, CD62L, and CD69. The CD8 MultiSort Kit has also been used for the isolation of IL-4–secreting CD8+ T cells in combination with the IL-4 Secretion Assay – Cell Enrichment and Detection Kit.1 |
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| Figure 1 |
| Isolation of human CD8+CD45RA+ cells from PBMCs using the CD8 MultiSort Kit and CD45RA MicroBeads. |
| PBMCs before separation |
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| CD8+ cells |
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| CD8+CD45RA+ |
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| Details |
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| Related products |
| CD16 MicroBeads, human (#130-045-701) |
| CD56 MicroBeads, human (#130-050-401) |
| CD25 MicroBeads II, human (#130-092-983) |
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| CD38 MicroBead Kit, human (#130-092-263) |
| CD62L MicroBeads, human (#130-091-758) |
| CD69 MicroBead Kit II, human (#130-092-355) |
| CD45RA MicroBeads, human (#130-045-901) |
| CD45RO MicroBeads, human (#130-046-001) |
| Anti-TCRγ/δ MicroBead Kit, human (#130-050-701) |
| CD8 antibodies |
| MACS® Cell Analysis Reagents |
| T Cell Activation/Expansion Kit, human (#130-091-441) |
| References |
| 1. Schwaiger et al. (2003) J. Immunol. 170: 613-619. |
| 2. Sauce et al. (2003) Blood 102: 1241-1248. |
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