| Description |
| The CD8 MultiSort Kit is a direct magnetic labeling system designed for the isolation of distinct CD8+ T and NK cell subsets from human PBMCs, bone marrow, cell cultures, or single-cell suspensions from tissue. The CD8+ cells are isolated with CD8 MultiSort MicroBeads. Then the magnetic particles are enzymatically released. The selected CD8+ cells can again be magnetically labeled and sorted for a second antigen. |
| Applications |
| The CD8 MultiSort Kit can be used to isolate specific CD8+ T cell or NK cell subsets. The selected CD8+ cell population can be sorted according to a second marker of interest, e.g., CD45RA, CD45RO, CD16, CD251, CD272, CD28, CD30, CD56, CD62L, and CD69. The CD8 MultiSort Kit has also been used for the isolation of IL-4–secreting CD8+ T cells in combination with the IL-4 Secretion Assay – Cell Enrichment and Detection Kit.1 |
| |
| Figure 1 |
| Isolation of human CD8+CD45RA+ cells from PBMCs using the CD8 MultiSort Kit and CD45RA MicroBeads. |
| PBMCs before separation |
 |
| CD8+ cells |
 |
| CD8+CD45RA+ |
 |
|
|
