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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
 
 
 
 
 
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD8 MicroBeads

CD8 MicroBeads

  • Unrivaled: excellent purity and unmatched recovery
  • Time-saving: cells can be immediately used for downstream applications
  • Reliable: proven in a plethora of publications
Description
CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8+ T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies1, and spleen.
Details
Background information
The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8+ cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8+ cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.


Downstream applications
Isolation or depletion of CD8+ cytotoxic T cells is performed in many different research fields such as infectious diseases, autoimmune diseases, allergy, and asthma, as well as tumor immunology.
Cytotoxic T cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays2,9 and cytotoxicity assays2,3, but also for the analysis of in vitro cytokine production4. CD8 MicroBeads have also been used for the depletion of CD8+ T cells from human PBMCs for immune reconstitution experiments in SCID mice.5
In combination with CD4 MultiSort MicroBeads, CD8 MicroBeads have been used for the isolation of CD4+CD8+ double-positive thymocytes.8
Other examples include various studies on viral infections, e.g., in vitro infections of CD8+ cells with HIV6, or monitoring of immune abnormalities in children of HIV-infected mothers7.
Columns
For positive selection: MS, LS, XS, or autoMACS Columns. For depletion: LD, CS, D, or autoMACS Columns.
 
Figure 1
Separation of CD8+ cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator.
PBMCs before separation
CD8- cells
CD8+ cells
Figure 2
Scanning electron micrograph of CD8+ T cells isolated using CD8 MicroBeads (courtesy of Prof. Peter Groscurth, Institute of Anatomy, University of Zürich, Switzerland).
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Details
Products
CD8 MicroBeads, human
- for 109 total cells
Download datasheet
130-045-201
Qty.:
 

CD8 MicroBeads, human – lyophilized
- for 109 total cells
Download datasheet
130-097-057
Qty.:
 

Related products
CD8 Antibodies
CD56 Antibodies
MACS® Cell Analysis Reagents
T Cell Activation/Expansion Kit, human (#130-091-441)
References
1. Akdis et al. (1999) J. Immunol. 163: 466-475.
2. Parra et al. (1996) J. Immunol. 158: 637-642.
3. Turner and Dockrell (1996) Immunology 87: 339-342.
4. Akdis et al. (1995) J. Immunol. Meth. 182: 251-261.
5. Walker et al. (1994) Immunolgy 83: 163-170.
6. Ennen et al. (1994) Proc. Natl. Acad. Sci. USA 91: 7207-7211.
7. Nielsen et al. (2001) Blood 98: 398-404.
8. Kutlesa et al. (2002) Immunology 105: 407-418.
9. Shanti et al. (2004) Immunology 112: 397-403.
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