| Description |
| The CD8+ Memory T Cell Isolation Kit was developed for the isolation of untouched CD8+CD45RO+CD45RA–CD56–CD57– memory T cells from human PBMCs. |
| Details |
Detailed separation procedure
Non-target cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD4, CD11c, CD14, CD15, CD16, CD19, CD34, CD36, CD45RA, CD56, CD57, CD61, CD123, CD141, TCR γ/δ, and CD235a (glycophorin A), and Anti-Biotin MicroBeads. Isolation of highly pure CD8+ memory T cells is achieved by depletion of the magnetically labeled cells.
Downstream applications
CD8+ memory T cells isolated with the CD8+ Memory T Cell Isolation Kit are used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on memory T cell activation. Untouched CD8+ memory T cells can also be used for phenotypical and functional studies. |
| |
| Figure 1 |
| Untouched CD8+ memory T cells were isolated from human PBMCs using the CD8+ Memory T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD45RA-FITC, CD57-PE, CD56-PE-Cy7, and CD8-APC. |
| Isolated CD8+ memory T cells |
| C |
 |
| D: Gated on CD8+CD56– cells. |
 |
| Before separation |
| A |
 |
| B: Gated on CD8+CD56- cells. |
 |
|
|
