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| CD4+ Effector Memory T Cell Isolation Kit |
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| Description |
| The Effector Memory CD4+ T Cell Isolation Kit was developed for the isolation of untouched memory T helper cells from human peripheral blood. |
| Details |
Detailed separation procedure
For magnetic depletion of non-target cells, PBMCs are incubated with a cocktail of biotinylated antibodies against CD45RA, CD8, CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, Anti-TCRγ/δ, and CD235a (glycophorin A) as well as APC-conjugated CD197 (CCR7) antibodies. In a second step, all antibody-labeled cells are labeled with MicroBead Cocktail for depletion. The CD4+ Effector Memory T Cell Isolation Kit provides a fast and efficient way to isolate untouched CD4+ effector memory T cells.
Downstream applications
CD4+ effector memory T cells isolated with the CD4+ Effector Memory T Cell Isolation Kit can be used for functional studies, for studies on signal transduction, or for analysis of cytokine expression. |
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| Figure 1 |
| Untouched CD4+ effector memory T cells were isolated from human PBMCs using the CD4+ Effector Memory T Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Cells were stained with CD4-FITC and CD45RO-PE. Cells were labeled with CD197 (CCR7)-APC during the separation procedure. |
| Before separation |
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| B: Gated on CD4+ cells. |
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| Isolated CD4+ effector memory T cells |
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| D: Gated on CD4+ cells. |
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| Details |
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| Products |
| CD4+ Effector Memory T Cell Isolation Kit, human |
- for 109 total cells
Download datasheet
130-094-125
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