CD294 (CRTH2) MicroBead Kit
Description The CD294 (CRTH2) MicroBead Kit, formerly known as Anti-CRTH2 MicroBead Kit, is an indirect magnetic labeling system for the enrichment of CD294+ cells. The CD294+ cells are fluorescently and indirectly magnetically labeled with CD294-PE and Anti-PE MicroBeads. CD294, also known as CRTH2, is a receptor for prostaglandin D2. CD294 is expressed on T helper type 2 (TH2) cells, a subpopulation of CD4+ T cells, but is not present on TH1 cells. CD294 is highly expressed on peripheral blood basophils and eosinophils. Furthermore, it is present on a small population of CD8+ T cells as well as on a subpopulation of dendritic cells. Applications The CD294 (CRTH2) MicroBead Kit can be used for positive selection or depletion of CD294+ cells. For isolation of the CD294+ T helper cell subset, the CD294 (CRTH2) MicroBead Kit can be combined with the CD4+ T Cell Isolation Kit II or the CD4 MultiSort Kit. A special protocol is available. Columns For positive selection: MS, LS, XS, or autoMACSâ„¢ Columns. For depletion: LD, CS, D, or autoMACS Columns. Further information Isolation of CD4+CD294 (CRTH2)+ Th2 cells [PDF; 161,5 KB]		Figure 1 Isolation of human peripheral blood CD4+CD294+ cells using the CD4+ T Cell Isolation Kit II and the CD294 (CRTH2) MicroBead Kit, an LD Column in a MidiMACSâ„¢ Separator, and two MS Columns in a MiniMACSâ„¢ Separator. PBMCs before separation Pre-enriched CD4+ T cells Isolated CD4+CD294+ TH2 cells

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