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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
 
 
 
 
 
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD294 (CRTH2) MicroBead Kit
Description
The CD294 (CRTH2) MicroBead Kit was developed for the positive selection or depletion of CD294+ cells. The CD294+ cells are fluorescently and indirectly magnetically labeled with CD294-PE and Anti-PE MicroBeads.
Details
Background information
CD294, also known as CRTH2, is a receptor for prostaglandin D2. CD294 is expressed on T helper type 2 (TH2) cells, a subpopulation of CD4+ T cells, but is not present on TH1 cells. CD294 is highly expressed on peripheral blood basophils and eosinophils. Furthermore, it is present on a small population of CD8+ T cells as well as on a subpopulation of dendritic cells.
For isolation of the CD294+ T helper cell subset, the CD294 (CRTH2) MicroBead Kit can be combined with the CD4+ T Cell Isolation Kit or the CD4 MultiSort Kit. A special protocol is available.
Columns
For positive selection: MS, LS, XS, or autoMACS Columns. For depletion: LD, CS, D, or autoMACS Columns.
Further information
Isolation of CD4+CD294 (CRTH2)+ Th2 cells from human PBMCs
[PDF; 265,3 KB]
 
Figure 1
Isolation of human peripheral blood CD4+CD294+ cells using the CD4+ T Cell Isolation Kit II and the CD294 (CRTH2) MicroBead Kit, an LD Column in a MidiMACS™ Separator, and two MS Columns in a MiniMACS™ Separator.
PBMCs before separation
Pre-enriched CD4+ T cells
Isolated CD4+CD294+ T cells
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Products
CD294 (CRTH2) MicroBead Kit, human
- for 109 total cells
Download datasheet
130-091-274
Qty.:
 

Related products
CD4 MultiSort Kit, human (#130-055-101)
CD4+ T Cell Isolation Kit, human (#130-096-533)
CD8 MultiSort Kit, human (#130-055-201)
CD8+ T Cell Isolation Kit, human (#130-096-495)
Pan T Cell Isolation Kit, human (#130-096-535)
CD4 antibodies
CD8 antibodies
MACS® Cell Analysis Reagents
T Cell Activation/Expansion Kit, human (#130-091-441)
References
1. Cosmi et al. (2000) Eur. J. Immunol. 30: 2972-2979.
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