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| Description |
| CD45RO MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs. |
| Details |
Background information
CD45RO is expressed on CD4+ and CD8+ T memory cells as well as on CD4+ effector T cells. CD45RO is also expressed on monocytes, macrophages, and granulocytes. The CD45RO antibody recognizes a 180 kDa isoform of the leukocyte common antigen (LCA).
CD45RO MicroBeads can be used for positive selection or depletion of CD45RO+ cells. For isolation of CD45RO+ T cell subsets, the CD45RO MicroBeads can be combined with CD4 and CD8 MACS® T Cell Isolation Kits1,2,7,8 or MultiSort Kits3,4. The CD45RO MicroBeads can also be used to isolate CD45RA+ cells from pre-sorted CD4+ or CD8+ T cells by depletion of CD45RO+ cells.5,7,8
Downstream applications
Cells isolated with CD45RO MicroBeads can be used for various studies, such as cytokine expression6 or receptor signaling. |
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| Figure 1 |
| CD4+ cells were enriched from PBMCs by using the CD4 MultiSort Kit. After release of the MultiSort MicroBeads, the cells were magnetically labeled with CD45RO MicroBeads. CD4+CD45RO+ effector and memory T helper cells were isolated by positive selection. |
| Pre-enriched CD4+ cells |
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| CD4+CD45RO+ cells |
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| References |
| 1. Krug et al. (2001) Eur. J. Immunol. 31: 3026-3037. |
| 2. Coccia et al. (1999) J. Biol. Chem. 274: 6698-6703. |
| 3. Wolthers et al. (1999) Blood 93: 1011-1019. |
| 4. Jonuleit et al. (2001) J. Exp. Med. 193: 1285-1294. |
| 5. Kimmig et al. (2002) J. Exp. Med. 195: 789-794. |
| 6. Malmberg et al. (2001) J. Immunol. 167: 2595-2601. |
| 7. Wagner et al. (2004) J. Immunol. 172: 954-963. |
| 8. Verhasselt et al. (2004) Eur. J. Immunol. 34: 762-772. |
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