| Description |
The CD4+ T Cell Isolation Kit has been developed for the faster isolation of untouched CD4+ T helper cells from human peripheral blood mononuclear cells (PBMCs).
This kit replaces the CD4+ T Cell Isolation Kit II (#130-091-155) |
| Details |
Detailed separation procedure
Non-CD4+ cells, i.e., CD8+ T cells, monocytes, neutrophils, eosinophils, B cells, dendritic cells, NK cells, granulocytes, γ/δ T cells, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD8, CD14, CD15, CD16, CD19, CD36, CD56, CD123, TCR γ/δ, and CD235a (Glycophorin A).
Subsequently, non-target cells are magnetically labelled with the
CD4+ T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.
Downstream applications
T helper cells isolated with the CD4+ T Cell Isolation Kit can be used when direct labeling of CD4+ T cell surface molecules could interfere with downstream applications, e.g., in studies on T helper cell activation.1–5 Untouched CD4+ T cells may also be used for the isolation of specific T helper cell subsets, such as naive6 or memory CD4+ T cells and activated CD4+ T cells. |
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| Figure 1 |
| Isolation of untouched CD4+ T cells from PBMCs using the CD4+ T Cell Isolation Kit, a LS Column, and a MidiMACS™ Separator. Cells were labeled with CD4-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. |
| PBMCs before separation |
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| Untouched CD4+ T helper cells |
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