| Description |
Next generation CD4+ T Cell Isolation Kit, human (# 130-096-533) available!
The CD4+ T Cell Isolation Kit II was developed for the isolation of untouched CD4+ T helper cells from human PBMCs. |
| Details |
Detailed separation procedure
Non-CD4+ T cells, i.e., CD8+ T cells, γ/δ T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are labeled by using a cocktail of biotin-conjugated antibodies against CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCR γ/δ, and CD235a (glycophorin A). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Thereby, highly pure CD4+ T cells are obtained.
Downstream applications
Untouched CD4+ T cells may also be used for the isolation of specific T helper cell subsets, such as naive1 or memory CD4+ T cells and activated CD4+ T cells. |
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| Figure 1 |
| Isolation of untouched CD4+ T cells from PBMCs using the CD4+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. |
| PBMCs before separation |
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| Untouched CD4+ T helper cells |
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