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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
 
 
 
 
 
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD4<sup>+</sup> T Cell Isolation Kit II

CD4+ T Cell Isolation Kit II

  • Unrivaled: excellent purity and unmatched recovery
  • Time-saving: cells can be immediately used for downstream applications
  • Reliable: proven in a plethora of publications
Description
Next generation CD4+ T Cell Isolation Kit, human (# 130-096-533) available!

The CD4+ T Cell Isolation Kit II was developed for the isolation of untouched CD4+ T helper cells from human PBMCs.
Details
Detailed separation procedure
Non-CD4+ T cells, i.e., CD8+ T cells, γ/δ T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are labeled by using a cocktail of biotin-conjugated antibodies against CD8, CD14, CD16, CD19, CD36, CD56, CD123, TCR γ/δ, and CD235a (glycophorin A). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. Thereby, highly pure CD4+ T cells are obtained.

Downstream applications
Untouched CD4+ T cells may also be used for the isolation of specific T helper cell subsets, such as naive1 or memory CD4+ T cells and activated CD4+ T cells.
Columns
MS, LS or autoMACS Columns.
Further information
Isolation of CD4+CD294 (CRTH2)+ Th2 cells from human PBMCs
[PDF; 265,3 KB]
Isolation of CD4+CD62L+ T cells from human PBMCs
[PDF; 302,7 KB]
 
Figure 1
Isolation of untouched CD4+ T cells from PBMCs using the CD4+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator.
PBMCs before separation
Untouched CD4+ T helper cells
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Details
Products
CD4+ T Cell Isolation Kit II, human
- for 109 total cells
Download datasheet
130-091-155
Qty.:
 

Related products
CD25 MicroBeads II, human (#130-092-983)
CD27 MicroBeads, human (#130-051-601)
CD69 MicroBead Kit II, human (#130-092-355)
CD45RA MicroBeads, human (#130-045-901)
CD45RO MicroBeads, human (#130-046-001)
CD294 (CRTH2) MicroBead Kit, human (#130-091-274)
Anti-TCRγ/δ MicroBead Kit, human (#130-050-701)
CD4 Antibodies
MACS® Cell Analysis Reagents
T Cell Activation/Expansion Kit, human (#130-091-441)
References
1. Verhasselt et al. (2004) Eur. J. Immunol. 34: 762-772.
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