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| Description |
| The CD4 MultiSort Kit was developed for the isolation of CD4+ T cell subsets by sequential sorting from human peripheral blood, bone marrow, cell cultures, or single-cell suspensions from tissue. |
| Details |
Detailed separation procedure
The CD4+ cells are isolated with CD4 MultiSort MicroBeads and the magnetic particles are then enzymatically released. The selected CD4+ T helper cells can again be magnetically labeled and sorted for a second antigen.
Downstream applications
The CD4 MultiSort Kit can be used to isolate specific CD4+ T cell subsets. The selected CD4+ T helper cell population can be sorted according to a second marker of interest, e.g., CD45RA1,2,3, CD45RO2,4, CD251,6, CD27, CD28, CD30, CD62L, and CD69. The CD4 MultiSort Kit has also been used for the isolation of CD4+CD8+ double-positive thymocytes in combination with CD8 MicroBeads.5 |
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| Figure 1 |
| Step-by-step CD4 MultiSort separation procedure. |
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| Details |
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| References |
| 1. Jonuleit et al. (2001) J. Exp. Med. 193: 1285–1294. |
| 2. Wolthers et al. (1999) Blood 93: 1011–1019. |
| 3. Mattila et al. (2000) Int. Immunol. 12: 1235–1243. |
| 4. Kimmig et al. (2002) J. Exp. Med. 195: 789–794. |
| 5. Kutlesa et al. (2002) Immunology 105: 407–418. |
| 6. Manigold et al. (2006) Blood 107: 4424–4432. |
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