| Description |
| The CD4 MultiSort Kit is a direct magnetic labeling system designed for the isolation of distinct CD4+ T cell subsets from human peripheral blood, bone marrow, cell cultures, or single-cell suspensions from tissue. The CD4+ cells are isolated with CD4 MultiSort MicroBeads and the magnetic particles are then enzymatically released. The selected CD4+ T helper cells can again be magnetically labeled and sorted for a second antigen. |
| Applications |
| The CD4 MultiSort Kit can be used to isolate specific CD4+ T cell subsets. The selected CD4+ T helper cell population can be sorted according to a second marker of interest, e.g., CD45RA1,2,3, CD45RO2,4, CD251,6, CD27, CD28, CD30, CD62L, and CD69. The CD4 MultiSort Kit has also been used for the isolation of CD4+CD8+ double-positive thymocytes in combination with CD8 MicroBeads.5 |
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| Figure 1 |
| Step-by-step CD4 MultiSort separation procedure. |
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