| Description |
Whole Blood CD4 MicroBeads were developed for automated positive selection of CD4+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD4 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator.
Whole Blood CD4 MicroBeads recognize about 65% of all peripheral blood T cells. The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4+ T helper cells. |
| Applications |
Whole Blood MicroBeads and the autoMACS™ Pro Separator or the autoMACS Separator allow fast sample processing under standardized conditions. This combination further allows easy and safe handling of hazardous samples, e.g., in HIV research. The purified CD4+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2.
For more information on MACS® Whole Blood MicroBeads click here. |
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| Figure 1 |
| CD4+ cells were separated from whole blood using Whole Blood CD4 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were fluorescently stained with CD4-PE and CD14-FITC. |
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| Figure 2 |
| Cell isolation by using Whole Blood MicroBeads and the autoMACS™ Pro Separator or the autoMACS Separator. |
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