CD4 MicroBeads

Unrivaled: excellent purity and unmatched recovery
Time-saving: cells can be immediately used for downstream applications
Reliable: proven in a plethora of publications

Description

CD4 MicroBeads were developed for the positive selection or depletion of CD4⁺ cells by direct magnetic labeling. The most commonly used cell sources are peripheral blood, leukapheresis¹, and cord blood. In addition, T helper cells have also been isolated from lymph nodes, thymus, spleen, skin biopsies², synovial fluids, and cell cultures.

Details

Background information
CD4 is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimers on CD4⁺ T helper cells. CD4 is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 molecule is the receptor for the human immunodeficiency virus. The CD4 antibody recognizes most thymocytes and about 65% of all peripheral blood T cells.

Downstream applications
Isolation or depletion of CD4⁺ T helper cells is performed in many different fields of research, such as infectious diseases, autoimmune diseases, allergy, asthma, and tumor immunology. T helper cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays¹⁰ and cytotoxicity assays³, as well as for the generation of T cell lines⁹. CD4⁺ T cells purified with CD4 MicroBeads have also been cultured and analyzed for in vitro cytokine production.⁴ Furthermore, they have been used in co‑culture experiments, e.g., for allogeneic stimulation by dendritic cells¹ or for B cell activation².
T helper cells isolated by MACS^®Technology were also used for studies on viral infections, e.g., for in vitro infections of cells with HIV⁵, to determine the infectivity of HIV-infected follicular dendritic cells⁶, immune reconstitution after anti-viral therapy⁷, and for monitoring of immune abnormalities in children of HIV-infected mothers⁸.

Columns

For positive selection: MS, LS, XS, or autoMACS Columns. For depletion: LD, CS, D, or autoMACS Columns.

Further information

Isolation of CD4⁺ T helper cells from human PBMCs
[PDF; 63 KB]

Figure 1

Isolation of CD4⁺ cells from PBMCs using CD4 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Figure 2

CD4⁺ T helper cells isolated from PBMCs using CD4 MicroBeads. Cells were stained with CD4-FITC in parallel to magnetic labeling.

CD4<sup>+</sup> T helper cells isolated from PBMCs using CD4 MicroBeads. Cells were stained with <a href="/en/PG_595_212_CD4.aspx">CD4-FITC</a> in parallel to magnetic labeling.