Naive CD4+ T Cell Isolation Kit II
Description The Naive CD4+ T Cell Isolation Kit II provides a fast and efficient way to isolate untouched naive CD4+ T cells from human peripheral blood. It is the new generation of the Naive CD4+ T Cell Isolation Kit, for even higher purities of the isolated naive T helper cell population. For magnetic depletion of memory CD4+ T cells and non-CD4+ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. This product replaces the Naive CD4+ T Cell Isolation Kit, human (#130-091-894). Applications Naive CD4+ T cells isolated with the Naive CD4+ T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.1, 2 Untouched naive CD4+ T cells can also be used for further isolation of specific T helper cell subsets. For isolation of naive CD4+ recent thymic emigrants, click here. Columns LS or autoMACS™ Columns		Isolation of untouched naive CD4+ T helper cells from PBMCs using the Naive CD4+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies. Before separation Isolated untouched naive CD4+ T cells

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