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 - Unrivaled: excellent purity and unmatched recovery
- Time-saving: cells can be immediately used for downstream applications
- Reliable: proven in a plethora of publications
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| Description |
| The Naive CD4+ T Cell Isolation Kit II was developed for the isolation of untouched naive CD4+ T cells from human peripheral blood. |
| Details |
Detailed separation procedure
For magnetic depletion of memory CD4+ T cells and non-CD4+ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.
Downstream applications
Naive CD4+ T cells isolated with the Naive CD4+ T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.1, 2 Untouched naive CD4+ T cells can also be used for further isolation of specific T helper cell subsets.
For isolation of naive CD4+ recent thymic emigrants the CD4+ Recent Thymic Emigrant Isolation Kit, human is recommended. |
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| Isolation of untouched naive CD4+ T helper cells from PBMCs using the Naive CD4+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies. |
| Before separation |
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| Isolated untouched naive CD4+ T cells |
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| Details |
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| Products |
| Naive CD4+ T Cell Isolation Kit II, human |
- for 109 total cells
Download datasheet
130-094-131
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| References |
| 1. Shen et al. (2005) J. Immunol. 175: 4927–4934. |
| 2. Fernandez-Sesma et al. (2006) J. Virol. 80: 6295–6304. |
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