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 - Unrivaled: excellent purity and unmatched recovery
- Time-saving: cells can be immediately used for downstream applications
- Reliable: proven in a plethora of publications
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| Description |
Next generation Pan T Cell Isolation Kit, human (#130-096-535) available!
The Pan T Cell Isolation Kit II was developed for the isolation of untouched T cells from human PBMCs. |
| Details |
Detailed separation procedure
Non-T cells, i.e., B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are incubated with a cocktail of biotin-conjugated antibodies against CD14, CD16, CD19, CD36, CD56, CD123, and CD235a (glycophorin A). These cells are subsequently labeled with Anti-Biotin MicroBeads to be magnetically depleted. Thereby, highly pure T cells are obtained.
Downstream applications
T cells isolated with the Pan T Cell Isolation Kit II can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation.1, 2 T cells isolated with the Pan T Cell Isolation Kit II have also been used to generate antigen-specific T cell lines.3, 4
Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive5 or memory T cells and activated T cells. |
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| Figure 1 |
Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit II, an LS Column, and
a MidiMACS™ Separator. |
| PBMCs before separation |
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| Untouched T cells |
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| Figure 2 |
| Isolation of untouched T cells. |
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| Details |
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| Products |
| Pan T Cell Isolation Kit II, human |
- for 109 total cells
Download datasheet
130-091-156
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| References |
| 1. Wagner et al. (2004) J. Immunol. 172: 954-963. |
| 2. Finney et al. (2004) J. Immunol. 172: 104-113. |
| 3. Faure et al. (2004) Nature Immunol. 5: 272-279. |
| 4. Bukczynski et al. (2004) Proc. Natl. Acad. Sci. USA 101: 1291-1296. |
| 5. Hafsi et al. (2004) J. Immunol. 173: 1249-1257. |
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