Pan T Cell Isolation Kit II
Description The Pan T Cell Isolation Kit II is an indirect magnetic labeling system for the isolation of untouched T cells from human PBMCs. Non-T cells, i.e., B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythroid cells, are incubated with a cocktail of biotin-conjugated antibodies against CD14, CD16, CD19, CD36, CD56, CD123, and CD235a (glycophorin A). These cells are subsequently labeled with Anti-Biotin MicroBeads to be magnetically depleted. Thereby, highly pure T cells are obtained. Applications T cells isolated with the Pan T Cell Isolation Kit II can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation.1, 2 T cells isolated with the Pan T Cell Isolation Kit II have also been used to generate antigen-specific T cell lines.3, 4 Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive5 or memory T cells and activated T cells. Columns LS or autoMACSâ„¢ Columns.		Figure 1 Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit II, an LS Column, and a MidiMACSâ„¢ Separator. PBMCs before separation Untouched T cells Figure 2 Isolation of untouched T cells using the Pan T Cell Isolation Kit II.