| Description |
Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD3 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
Whole Blood CD3 MicroBeads recognize the CD3 antigen which is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes. |
| Applications |
Whole Blood MicroBeads allow the fast isolation of CD3+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS™ Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD3+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2.
For more information on MACS® Whole Blood MicroBeads click here. |
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| Figure 1 |
| CD3+ cells were separated from whole blood using Whole Blood CD3 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were fluorescently stained with CD3-FITC and CD45-PE. |
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