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 - Unrivaled: excellent purity and unmatched recovery
- Time-saving: cells can be immediately used for downstream applications
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| Overview |
| The Naive Pan T Cell Isolation Kit was developed for the fast and efficient isolation of untouched naive T cells from human PBMCs. |
| Details |
Detailed separation procedure
For depletion of memory T cells and non-T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD57, CD123, anti-HLA-DR, CD235a (Glycophorin A), and CD244 antibodies. Optionally, biotinylated Anti-TCRγ/δ antibodies can be added for depletion of TCRγ/δ+ T cells. The cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. CD61 MicroBeads are added for concurrent depletion of platelets.
Downstream applications
Naive pan T cells isolated with the Naive Pan T Cell Isolation Kit can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T cell activation and in functional studies of DCs. Untouched naive pan T cells can also be used for further isolation of specific T cell subsets. |
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| Figure 1 |
| Separation of untouched naive T cells from PBMCs by depletion of non-T cells using the Naive Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD3-VioBlue, CD197-PE and CD45RA-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. |
| PBMCs before separation |
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| Untouched naive T cells |
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| Favorites / Prices |
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| Details |
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| Products |
| Naive Pan T Cell Isolation Kit, human |
- for 109 total cells
Download datasheet
130-097-095
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| References |
| 1. Che et al. (2010) Eur. J. Immunol. 40: 2248-2252 |
| 2. Gattinoni et al. (2011) Nat. Med. 17: 1290-1297. |
| 3. Jedema et al. (2011) Haematologica 96 (8): 1204-1212. |
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