| Description |
The Pan T Cell Isolation Kit has been developed for the faster isolation of untouched T cells from human peripheral blood mononuclear cells (PBMCs).
This kit replaces the Pan T Cell Isolation Kit, II (#130-091-156) |
| Details |
Detailed separation procedure
Non-target cells, i.e., monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the Pan T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.
Downstream applications
T cells isolated with the Pan T Cell Isolation Kit can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation.1, 2 T cells isolated with the Pan T Cell Isolation Kit have also been used to generate antigen-specific T cell lines.3, 4 Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive5 or memory T cells and activated T cells. |
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| Figure 1 |
| Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD2-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. |
| PBMCs before separation |
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| Untouched T cells |
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| Figure 2 |
| Isolation of untouched T cells. |
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