| Description |
| CD138 MicroBeads have been developed for the isolation of plasma cells from human peripheral blood mononuclear cells (PBMCs), bone marrow, or lymphoid tissue. CD138, also known as syndecan-1, is expressed on normal and malignant human plasma cells and commonly used as a universal marker for their identification.1 CD138 is also expressed on basolateral surfaces of endothelial cells, embryonic mesenchymal cells, vascular smooth muscle cells, and neural cells.2,3 In mouse, CD138 is expressed on plasma cells but also B cell precursors in the bone marrow.4 |
| Applications |
| CD138 MicroBeads can be used for the isolation or depletion of plasma cells from PBMCs, bone marrow, leukaphereses harvests, or single-cell suspensions from lymphoid tissue, for phenotypical and functional characterization, for example, by flow cytometric analysis, molecular biology studies such as PCR, microarrays, or protein analysis. CD138 MicroBeads are also suitable for positive selection or depletion of malignant plasma cells from bone marrow of patients with multiple myeloma. In combination with the Inside Stain Kit CD138 MicroBeads can be used for positive selection and subsequent solid phase intracellular staining of normal and malignant plasma cells, for example, for kappa or lambda light chains of immunoglobulins. |
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| Figure 1 |
| Isolated CD138+ plasma cells from bone marrow of a patient with multiple myeloma. Cells were isolated by MACS® Technology and intracellularly stained for immunoglobulin kappa light chains. |
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| Figure 2 |
| Bone marrow mononuclear cells (BM MNCs) from a patient with multiple myeloma were separated using the CD138 MicroBeads, an MS Column, and a MiniMACS Separator. Cells are fluorescently stained with CD138-PE. |
| BM MNCs before separation |
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| CD138– cells |
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| CD138+ cells |
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