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| Anti-Melanoma (MCSP) MicroBeads |
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| Overview |
Anti-Melanoma (MCSP) MicroBeads were developed for
- enrichment of disseminated melanoma cells from peripheral blood1, bone marrow, leukapheresis harvest, and lymphoid tissue of melanoma patients—even in early disease stages2,3,
- isolation of melanoma cells from single-cell suspensions of primary tumor tissue or primary skin cell culture.
Anti-Melanoma MicroBeads can be used in combination with the Inside Stain Kit. This enables the in-column staining of enriched melanoma cells for intracellular antigens, e. g., Melan-A. By performing combined enrichment and staining, a sensitivity of melanoma cell detection of one melanoma cell in 2×107 PBMC background cells can be achieved4.
For convenient subsequent immunocytochemical detection the Melanoma Enrichment and Detection Kit can be used. |
| Details |
Background information
The antibody clone 9.2.27 recognizes the melanoma-associated chondroitin sulfate proteoglycan (MCSP) antigen, also known as high molecular weight melanoma-associated antigen, or NG2. MCSP is expressed on melanoma cells but not on carcinoma cells, fibroblastoid cells, or cells of hematopoietic origin.
Downstream applications
Cells enriched with Anti-Melanoma (MCSP) MicroBeads can be analyzed by immunocytochemistry, flow cytometry, or molecular biology methods. The isolated cells can also be cultured. |
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| Figure 1 |
| Melanoma cells from peripheral blood of a malignant melanoma patient isolated with Anti-Melanoma (MCSP) MicroBeads. Cells were stained according to the in-column, intracellular staining protocol using the Inside Stain Kit in combination with an anti-Melan A antibody and alkaline phosphatase. |
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| Details |
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| Products |
| Anti-Melanoma (MCSP) MicroBeads, human |
| For research use only |
- for 109 total cells
Download datasheet
130-090-452
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| References |
| 1. Benez et al. (1999) J. Clin. Lab. Anal. 13: 229-233. |
| 2. Ulmer et al. (2008) Clin. Cancer Res. 14: 4469–4474. |
| 3. Ulmer et al. (2004) Clin. Cancer Research 10: 531–537. |
| 4. Siewert et al. (2000) Recent Results Cancer Res. 158: 51–60. |
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