| Description |
| Anti-Melanoma (MCSP) MicroBeads were developed for enrichment of disseminated melanoma cells from peripheral blood, bone marrow, and lymphoid tissue of melanoma patients—even in early disease stages4. They are also used for the purification of melanoma cells from single cell suspensions of primary tumor tissue. The antibody clone 9.2.27 recognizes the melanoma-associated chondroitin sulfate proteoglycan (MCSP) antigen, also known as high molecular weight melanoma-associated antigen, or NG2. MCSP is expressed on melanoma cells but not on carcinoma cells, fibroblastoid cells, or cells of hematopoietic origin. To block Fc receptor–mediated non-specific labeling of non-epithelial cells, it is strongly recommended to use FcR Blocking Reagent (human) before magnetic labeling. |
| Applications |
Cells enriched with Anti-Melanoma (MCSP) MicroBeads can be analyzed by immunocytochemistry, flow cytometry, or molecular biology methods. The isolated cells can also be cultured.
Anti-Melanoma MicroBeads can be used in combination with the Inside Stain Kit. This enables the in-column staining of enriched melanoma cells for intracellular antigens, e. g., Melan A.
By performing combined enrichment and staining, a sensitivity of melanoma cell detection of 10–7 can be achieved. |
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| Figure 1 |
| Melanoma cells from peripheral blood of a malignant melanoma patient isolated with Anti-Melanoma (MCSP) MicroBeads. Cells were stained according to the in-column, intracellular staining protocol. The Inside Stain Kit was employed in combination with an anti-Melan A antibody and alkaline phosphatase. |
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