| Description |
The Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry is a detection system that allows a more than 10,000-fold enrichment and the subsequent immunocytochemical detection of disseminated epithelial tumor cells. The ready-to-use kit provides all necessary reagents for the magnetic enrichment and immunocytochemical detection of enriched tumor cells.
The carcinoma cells are permeabilized, fixed, and magnetically labeled with Anti-Cytokeratin MicroBeads. Magnetically labeled cells are then stained with a highly specific Anti-Cytokeratin-FITC antibody and Anti-FITC Alkaline Phosphatase and subjected to magnetic separation. After separation, cells are spun down onto a glass slide and stained with an alkaline phosphatase substrate. |
| Applications |
| The optimized enrichment and detection system allows the highly specific enrichment and subsequent detection of disseminated tumor cells in blood, bone marrow, or lymphoid tissue with sensitivities below 10–7. |
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| Figure 1 |
| Immunocytochemical detection of breast cancer cells enriched using MACS® Technology and stained with Anti-Cytokeratin-FITC and Anti-FITC-Alkaline Phosphatase plus substrate. |
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