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| Carcinoma Cell Enrichment and Detection Kit - Immunocytochemistry |
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| Overview |
| The Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry is a ready-to-use kit that allows for more than a 10,000-fold enrichment and the subsequent immunocytochemical detection of disseminated epithelial tumor cells from blood, bone marrow, or lymphoid tissue by the positive selection of cytokeratin 7/8 expressing cells. |
| Details |
Background information
Most malignant cells which have their origin in epithelial tissue express cytokeratin and can be recognized by an anti-cytokeratin antibody1. With the Carcinoma Cell Enrichment and Detection Kit, disseminated epithelial tumor cells can rapidly and efficiently be enriched using MACS Cytokeratin MicroBeads, e.g. from a frequency of 1 tumor cell in 107 leukocytes to a frequency of 1 tumor cell per 103 leukocytes. Thus, large patient samples can be narrowed and screened for disseminated tumor cells on only a few microscopy slides.
Detailed procedure
The carcinoma cells are permeabilized, fixed, and magnetically labeled with Anti-Cytokeratin MicroBeads. Magnetically labeled cells are then stained with a highly specific Anti-Cytokeratin-FITC antibody and Anti-FITC Alkaline Phosphatase and subjected to magnetic separation. After separation, cells are spun down onto a glass slide and stained with an alkaline phosphatase substrate. |
| Columns |
| For enrichment and in-column staining: MS Columns. |
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| Figure 1 |
| Immunocytochemical detection of breast cancer cells enriched using MACS® Technology and stained with Anti-Cytokeratin-FITC and Anti-FITC-Alkaline Phosphatase plus substrate. |
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| Favorites / Prices |
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| Details |
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| Products |
| Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human |
| For research use only |
- for 1-3×109 total cells
- for up to 20 tests
Download datasheet
130-060-301
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| References |
| 1. Heatley et al. (1995) J. Clin. Pathol. 48: 26-32. |
| 2. Martin et al. (1998) Exp. Hematol. 26: 252–264. |
| 3. Gaforio et al. (2003) Int. J. Cancer 107: 984–990. |
| 4. Campos et al. (2008) J. Histochem. Cytochem. 56: 667–675. |
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