| Description |
Anti-ErbB-2 MicroBeads can be used for the enrichment of disseminated epithelial ErbB-2-expressing tumor cells from blood, bone marrow, or leukapheresis harvest. ErbB-2, also known as HER2/neu, encodes the human epidermal growth factor receptor which is overexpressed in 25–30% of breast cancer patients.
In order to prevent Fc receptor–mediated non-specific labeling of non-epithelial cells, it is strongly recommended to use FcR Blocking Reagent (human) before magnetic labeling. |
| Applications |
| Enriched ErbB-2+ cells can be characterized by using different techniques such as quantitative real-time PCR1, immunocytochemistry, or flow cytometry. The enriched cells can be taken into culture. |
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| Figure 1 |
| A cluster of cytokeratin-positive cells isolated with Anti-ErbB-2 MicroBeads from peripheral blood of a patient with primary breast cancer. Cells were stained following the in-column intracellular staining protocol using the Inside Stain Kit in combination with Anti-Cytokeratin-Alkaline Phosphatase. |
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