| Overview |
CD326 (EpCAM) MicroBeads can be used for the:
- positive isolation or depletion of epithelial cells or epithelial tumor cells from single-cell preparations of tissues and organs,
- enrichment of disseminated epithelial tumor cells expressing CD326 from peripheral blood, bone marrow, or even lymph nodes, serous effusions, or stool samples1 of patients with carcinomas,
- positive selection of functional pluripotent hepatic progenitor cells (hepatic stem cells and hepatoblasts) from fetal, neonatal, pediatric, and adult liver tissue2,3.
- positive isolation of tumor exosomes from peripheral blood4,
- enrichment of exfoliated epithelial cells from gastric aspirates and stool samples of preterm infants5,
- purification of primary tumor cell cultures6.
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| Details |
Background information
CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) expression is seen in the majority of carcinomas. It is expressed on cells of epithelial origin, epithelium-derived tumor cells7, circulating tumor cells, and cancer stem cells.
Downstream applications
Tumor cells enriched by CD326 (EpCAM) MicroBeads can be cultured8 and analyzed by immunocytochemistry9, flow cytometry, or molecular biology techniques, for example, RT-PCR10. |
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| Figure 1 |
CD326 (EpCAM)+ tumor cells isolated from pleural effusion of a patient with adeno-carcinoma using CD326 (EpCAM) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD326 (EpCAM)-FITC and Anti-FITC Alkaline Phosphatase plus substrate.
(Courtesy of Dr. Motherby and Prof. Böcking, Inst. of Cytopathol., Univ. Düsseldorf, Germany.) |
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| Figure 2 |
| Enrichment of tumor cells from PBMCs spiked with cells of the breast cancer cell line BT474. Separation was performed using an MS Column and CD326 (EpCAM) MicroBeads. |
| A: Cell mixture before separation (6×107 PBMCs were mixed with 50,000 tumor cells) |
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| B: Positive fraction with enriched breast cancer cells (1.54×105 total cells) |
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