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  • MACS® Cell Analysis
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
 
 
 
 
 
 
 
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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CD326 (EpCAM) MicroBeads
Overview
CD326 (EpCAM) MicroBeads can be used for the:
  • positive isolation or depletion of epithelial cells or epithelial tumor cells from single-cell preparations of tissues and organs,
  • enrichment of disseminated epithelial tumor cells expressing CD326 from peripheral blood, bone marrow, or even lymph nodes, serous effusions, or stool samples1 of patients with carcinomas,
  • positive selection of functional pluripotent hepatic progenitor cells (hepatic stem cells and hepatoblasts) from fetal, neonatal, pediatric, and adult liver tissue2,3.
  • positive isolation of tumor exosomes from peripheral blood4,
  • enrichment of exfoliated epithelial cells from gastric aspirates and stool samples of preterm infants5,
  • purification of primary tumor cell cultures6.
Details
Background information
CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA) expression is seen in the majority of carcinomas. It is expressed on cells of epithelial origin, epithelium-derived tumor cells7, circulating tumor cells, and cancer stem cells.

Downstream applications
Tumor cells enriched by CD326 (EpCAM) MicroBeads can be cultured8 and analyzed by immunocytochemistry9, flow cytometry, or molecular biology techniques, for example, RT-PCR10.
Columns
MS, LS, XS Columns, or autoMACS Columns.
Further information
Cancer Research Brochure
[PDF; 4,3 MB]
 
Figure 1
CD326 (EpCAM)+ tumor cells isolated from pleural effusion of a patient with adeno-carcinoma using CD326 (EpCAM) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were stained with CD326 (EpCAM)-FITC and Anti-FITC Alkaline Phosphatase plus substrate.
(Courtesy of Dr. Motherby and Prof. Böcking, Inst. of Cytopathol., Univ. Düsseldorf, Germany.)
Figure 2
Enrichment of tumor cells from PBMCs spiked with cells of the breast cancer cell line BT474. Separation was performed using an MS Column and CD326 (EpCAM) MicroBeads.
A: Cell mixture before separation (6×107 PBMCs were mixed with 50,000 tumor cells)
B: Positive fraction with enriched breast cancer cells (1.54×105 total cells)
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Products
CD326 (EpCAM) MicroBeads, human
For research use only
- for 109 total cells
Download datasheet
130-061-101
Qty.:
 

CD326 (EpCAM) MicroBeads + CD326 (EpCAM)-PE, human
For research use only
- for 109 total cells
- for 100 tests with 107 total cells
Download datasheet #1
Download datasheet #2
130-092-234
Qty.:
 

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FcR Blocking Reagent, human (#130-059-901)
CD326 (EpCAM) Antibodies
CD326 (EpCAM) Tumor Cell Enrichment and Detection Kit
Anti-ErbB-2 MicroBeads, human (#130-090-482)
ErbB-2 Tumor Cell Enrichment and Detection Kit
Carcinoma Cell Enrichment Kit
Carcinoma Cell Enrichment and Detection Kit - Immunocytochemistry
Anti-Cytokeratin-Alkaline Phosphatase, human (#130-090-462)
Anti-Cytokeratin (CK3-6H5) Antibodies
Anti-Cytokeratin (CK3-3E4) Antibodies
Inside Stain Kit (#130-090-477)
CD45 MicroBeads, human (#130-045-801)
CD45 Antibodies
References
1. Lewin et al. (2006) Cancer Res. 66: 1859–1865.
2. Schmelzer et al. (2006) Stem Cells 24: 1852–1858.
3. Schmelzer et al. (2007) J. Exp. Med. 204:1973-1987.
4. Taylor et al. (2008) Gynecol. Oncol.110: 13–21.
5. Kaeffer et al. (2007) Pediatr. Res. 62: 564–569.
6. Chan et al. (2006) Clin. Cancer Res. 12: 1859–1867.
7. Moldenhauer et al. (1987) Br. J. Cancer 56: 714–721.
8. Leinung et al. (2000) Langenbeck´s Arch. Surg. 385: 337–343.
9. Krüger et al. (2000) Transfusion 40: 1489–1493.
10. Molloy et al. (2008) Breast Cancer Res. Treat. 112: 297–307.
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