From protein regulation to diseases
The Nobel Prize-honored ubiquitin-proteasome system (UPS) establishes the major pathway for controlled protein degradation: It recognizes and destructs ubiquitin-tagged proteins. The UPS affects numerous processes, including cell cycle, DNA repair, and signal transduction. Any deregulation of this system – and its protein quality control – is associated with diseases, ranging from cancer to neurodegeneration.
Investigate UPS components and novel candidate genes!
Disease-related changes within the UPS can be monitored and the impact of drug treatment on UPS components analyzed with the PIQORâ„¢ Ubiquitin-PS Microarrays (see fig. 1). Also, the tissue-specificity of the UPS components can be characterized.
Various microarray applications
The PIQOR Ubiquitin-PS Microarray contains about 1,250 genes and allows the detection of mRNA expression changes along the entire ubiquitin-proteasome pathway. In addition, it contains a large number of unpublished candidate genes, identified using homology-based profile techniques, protein interaction analyses, and in cooperation with distinguished experts1-7.
PIQOR Ubiquitin-PS Microarray is available as a convenient microarray kit or within the scope of Microarray Service.
Please see download area for "Further information".
On request - please inquire delivery time. |
| Gene Overview |
Human: 1,278 genes
Mouse: 1,250 genes
Genes are spotted in quadruplicates.
Ubiquitin-activating (E1) and Ubiquitin-conjugating enzymes (E2)
Ubiquitin ligases (E3): HECT and RING-finger types, A20, etc.
Components of complex E3 ligases: e.g. SCF, APC, CBC, SOCS, BTB
Deubiquitylating enzymes: UCH, USP, OTU, JAMM, Josephin, etc.
Ubiquitin recognition components: UBA, UIM, CUE, NZF, UEV, PAZ, UBM, UBZ, GAT, etc.
Analogous enzymes and recognition components for ubiquitin-like proteins: SUMO, Nedd8, ISG15, Fat10, etc.
Proteasome components and associated factors
Regulators of the immunoproteasome
Components and regulators of the CSN signalosome
Proteins with internal ubiquitin-like domains
UPS-associated protein quality control components: HSPs and J-domain proteins, etc.
Other UPS-associated factors: protein interactors of UPS components, etc. |
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| Figure 1 |
Gene expression analysis of three human cell lines treated with the proteasomal inhibitor MG132 using PIQORâ„¢ Ubiquitin-PS Microarrays. Jurkat, Raji and HEK293 cells were harvested after 1 h, 4 h and 24 h, respectively.
Cluster analysis reveals up-regulated 26S-proteasomal subunits, relevant for the degradation of misfolded proteins (19S regulatory particle and β-subunits). The βi-subunits and the subunits of the PA28 regulator, specific to the immunoproteasome, are down-regulated. |
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Gene expression:
red = increase;
green = decrease;
black = no alteration;
gray = no signal detection.
x-axes: array experiments;
y-axes: gene names. |
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