The PIQOR™ Stem Cell Microarrays consist of relevant marker genes for human and murine stem cells as well as key genes involved in their differentiation. Genes have been assembled in cooperation with experts in the field following extensive literature screens, gene expression profiling data, and bioinformatics based homology screens.
Gene expression experiments allow quality control of different stem cell types, comparison of different stages, and optimization of differentiation protocols.
The PIQOR Stem Cell Microarrays are available as a convenient microarray kit or within the scope of Microarray Service.
Please see download area for "Further information"
On request - please inquire for delivery time. |
| Gene overview |
Human: 942 genes
Mouse: 916 genes
Genes are spotted in quadruplicates.
Stem cell quality control using gene expression patterns
Analysis of positive and negative marker genes allows quality control of embryonic and hematopoietic stem cells, and marrow stromal cells (MSCs).
Human embryonic stem cells
NANOG, POU5F (Oct4), TERT, ZFP42, etc.
Human hematopoietic stem cells
CD34, CD133, CD117, etc.
Human marrow stromal cells (MSCs)
CD166, CD44 isoforms, CD73, CD90, CD271 (LNGFR), etc.
2. Evaluation of stem cell differentiation
The tracking of stem cell differentiation by gene expression profiling allows the comparison of different stages, optimization of differentiation protocols, or characterization of differentiation end points.
Ectoderm
FGF5, NEFL, PAX6, POU3F2, etc.
Mesoderm
BMP2, BMP4, BRACHYURY, CD34, NOGGIN, SCX, etc.
Endoderm
GATA2, GATA5, HNF3B, HNF4A, IPF1, etc.
Connective and adipose tissue
Adipocytes: HMGIC, OB, PPARG, PARG2, etc.
Chondrocytes: CSPG1, ASPIC1, etc.
Osteoblasts: CBFA1, BGLAP, etc.
Cardiovascular system
CACNA1A, CSX, GATA4, GJA1, GJA5, GJA7, MYH6, MYL2, NPPA, TNNC1, TNNT2, etc.
Liver
AFP, ALB, CYP2C9, CYP3A4, CYP3A7, HNF3B, HNF3G, HNF4A, KRT18, KRT8, PCK1, SERPINA1, TTR, etc.
Nervous system
Astrocytes: EAAT1, FABB, GFAP, S100B, etc.
Microglia: CD68, etc.
Neuronal precursors: APOER2, ATH1, ATH4, CD24, DLX1, DLX2, DLX5, NES, etc.
Neurons: APOER2, ASCL1, CD24, DLX1, DLX2, DLX5, etc.
Oligodendrocytes: CNP, OLIG1, OSP, PLP, etc.
Skin
ITGB1, ITGA6, CTNNB1, K15, MCSP, etc.
Six housekeeping genes and six controls
ACTA2, CYPA, GAPDH, HPRT, TUBA, TUBB;
Herring sperm DNA, salt, four artificial control RNAs |
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| Figure 1 |
| PIQOR Stem Cell Microarray |
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