Whole Blood CD138 MicroBeads ( #130-093-062 )

Whole Blood CD138 MicroBeads

Description

Whole Blood CD138 MicroBeads were developed for rapid positive selection of CD138⁺ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD138 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit.
The CD138 antigen, also known as syndecan-1, is primarily expressed on normal and malignant plasma cells in the bone marrow. In peripheral blood of healthy donors approximately 50% of plasma cells express this antigen. However, CD138 is not expressed by naive B cells, germinal center B cells, memory B cells, T cells, or monocytes.

Applications

Whole Blood MicroBeads allow the fast isolation of CD138⁺ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS™ Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD138⁺ cells are well-suited for flow cytometric, functional, or molecular analysis.
For more information on MACS® Whole Blood MicroBeads click here.

Columns

autoMACS Columns: autoMACS™ Pro Separator (Program Posselwb; 0.25–15 mL sample volume) or autoMACS Separator Program Posseld2; 0.25–3 mL sample volume)
Whole Blood Column Kit: for 0.25–15 mL sample volume

Figure 1

CD138⁺ cells were separated from whole blood using Whole Blood CD138 MicroBeads and the autoMACS™Pro Separator with program Posseldwb. Cells were fluorescently stained with CD138-PE and CD19-APC.

Isolated CD138⁺ cells